專任師資

葉靖輝 博士
葉靖輝 博士 職稱: 副教授
學歷: 國立台灣大學植物所
專長: 植物逆境生理學、蛋白質體學、植物組織培養、訊息傳遞
信箱: yehch63@cc.ncu.edu.tw
分機: 65068, 65078

個人介紹

  1. 職 稱:副教授
  2. 學 歷:
    1. 09/1994 ~ 06/1999 Department of Botany, National Taiwan University. PhD Degree.
    2. 09/1983 ~ 06/1987 Department of Botany, National Taiwan University, B.S. Degree.
  3. 經 歷:
    1. 03/2002 ~07/2003 Dr. Tuan-Hua David Ho’s Lab Postdoctoral Research Fellow, Department of Biology, Washington University in St. Louis
    2. 02/2001 ~ 07/2001 Adjunct Assistant Professor, Department of Life Sciences, TzuChi University.
    3. 08/1999 ~ 01/2002 Dr. Chu-Yung Lin’s Lab Postdoctoral Research Fellow, Department of Botany, National Taiwan University.
  4. 現 職: 2015/8-present 國立中央大學 生命科學研究所 副教授
  5. 聯絡方式:(03)422-7151 (分機 65068)
  6. FAX:(03) 422-8482
  7. E-mail:yehch63@cc.ncu.edu.tw

授課

  1. 普通生物學
  2. 植物學
  3. 專題討論
  4. 高等植物生理
  5. 專題研究植物生物化學
  6. 植物細胞培養技術
  7. 植物生理逆境
  8. 植物荷爾蒙
  9. 植物生長與發育
  10. 植物的基因轉型作用
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研究領域

植物因無法移動,因此需以不同於動物的方式適應各式各樣的環境逆境。由於溫室效應的影響地球的溫度變異越來越大,連帶使台灣整體氣候發生改變,四季溫差愈趨明顯,乾旱的問題亦伴隨而來,使得在田間栽種的農作物,皆有可能遭受到不同程度的熱、低溫或乾旱等環境逆境的傷害(Yeh et al., 2012)。當植物處於較高環境溫度時,除成熟的組織、種子或花粉能忍受此逆境外,高溫會使得大部分生長中的植物組織停止生長甚至死亡。根據現有高溫逆境產生效應時間區分,高溫誘導效應可分成早期反應(Early response)與晚期反應(Late response) (圖一)。早期反應:葉溫因環境高溫而迅速上昇,致使蒸散速率加快與水分散失增加、光合作用與呼吸作用效率降低、膜穩定度性下降和自由基大量累積;晚期反應:包括蛋白質變性量增加,誘導細胞保護分子合成量或累積量增加(如小分子量熱休克蛋白質與SOD等),降低細胞傷害。上述反應中,蒸散作用降溫與保護分子累積皆被視為減少細胞受熱逆境傷害的重要機制。

圖(一) 植物高溫逆境誘導效應。

水稻可謂我國現今最主要的糧食作物,同時它亦提供全球超過1/2人口的食物來源,因此如何減低環境對水稻生長的傷害以提高稻米產量實為現今重要的研究課題。溫度為影響水稻栽培與生產之重要因子,特別是高溫對水稻的生長與結穗會產生相當程度的影響。因此我們實驗室即針對水稻熱逆境相關基因功能進行分析,主要研究方向分成下列二部分:

第一部分: 探討水稻氣孔蒸散作用基因-OsRZFP34對水稻耐熱性及水稻產量的影響
利用寡核苷酸DNA微陣列篩到一個分別可受高溫及ABA誘導表現的水稻RING zinc-finger基因,命名為OsRZFP34。實驗發現其大量表現可在水稻中增加葉片氣孔的開闔程度,反之在降低表現量的突變株中,正常情況下葉片氣孔開闔程度即有明顯減少情形。熱逆境下,OsRZFP34大量表現轉植株較野生型植株具有較高降溫效果與蒸散速率(圖二)。進一步研究顯示OsRZFP34可調控保衛細胞中鈣離子濃度,影響水稻氣孔蒸散作用。現正分析OsRZFP34表現對水稻產量的影響。

() 水稻野生型(WT)OsRZFP34過量表現型(OsOE1, OsOE2, OsOE3)OsRZFP34缺失表現型(osrzfp34)高溫下葉溫分析

第二部分: 分析小分子量熱休克蛋白質基因-OsHSP16.9A生理功能
小分子量熱休克蛋白質普遍存在於原核與真核生物中,它們被視為與生物耐熱性有密切關聯性。為研究水稻小分子量熱休克蛋白質OsHSP16.9A的生理功能,我們建立OsHSP16.9A大量表現(OsHSP16.9A-OE)和降低表現(OsHSP16.9A-i)的水稻轉殖株。水稻性狀分析發現OsHSP16.9A過量表現可降低水稻種子的白堊質發生情形,提高水稻種子、幼苗和花粉耐熱性 (圖三)。此顯示OsHSP16.9A與水稻在高溫下的耐熱性及白堊質有密切關係。

() 野生型水稻(TNG67)OsHSP16.9A過量表現水稻轉殖株(Oshsp16.9A-OE)OsHSP16.9A缺失表現水稻轉殖株(Oshsp16.9A-RNAi)與小分子量熱休克蛋白質缺失表現水稻轉殖株(Oshsp-RNAi)分析

研究計畫

  • 2015/08/01~2018/07/31
    Grant: NTD 3,360,000
    OsRZFP34參與之蒸散作用與其在水稻耐熱性角色之研究 (MOST 104-2313-B-008-001-MY3) 主持人
  • 2014/08/01~2015/07/31
    Grant: NTD 1,080,000
    受熱與ABA誘導表現之水稻RING zinc-finger蛋白質基因OsRZFP34生理功能分析 (NSC 103-2313-B-008-001-) 主持人
  • 2013.08.01 ~ 2014.07.31
    Grant: NTD 880,000
    環境逆境下水稻熱休克轉錄因子差異性調控機制分析 (NSC 102-2313-B-008-001-) 主持人
  • 2012.08.01 ~ 2013.12.31
    Grant: NTD 800,000
    改善水稻耐熱性並降低穀粒白堊質技術之開發-〈子計畫三〉熱誘導蛋白質與水稻稔實率、白堊質關聯性之研究 (NSC 101-2324-B-008-002-) 主持人
  • 2012.08.01 ~ 2013.07.31
    Grant: NTD 850,000
    逆境下水稻熱休克轉錄因子差異性誘導機制分析 (NSC 101-2313-B-008-001-) 主持人
  • 2010.08.01 ~ 2012.07.31
    Grant: NTD 2,400,000
    水稻耐高溫逆境突變株之篩選、建立與應用 (NSC 99-2324-B-008-002-MY2) 主持人

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論文發表

Refereed papers

  1. Hsu KH, Liu CC, Wu SJ, Kuo YY, Lu CA, Wu CR, Lian PJ, Hong CY, Ke YT, Huang JH, Yeh CH* (2014) Expression of a gene encoding a rice RING zinc‑finger protein, OsRZFP34, enhances stomata opening. Plant Molecular Biology 86, 125-137. (SCI) (IF=4.072, Ranking 19/196) (corresponding author)
  2. Chou WL, Huang LF, Fang JC, Yeh CH, Hong CY, Wu SJ, Lu CA* (2014) Divergence of the expression and subcellular localization of CCR4‑associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Molecular Biology 85, 443-458. (SCI) (IF=4.072, Ranking 19/196)
  3. Wang LC, Wu JR, Chang WL, Yeh CH, Ke YT, Lu CA, Wu SJ* (2013) Arabidopsis HIT4 encodes a novel chromocentre-localized protein involved in the heat reactivation of transcriptionally silent loci and is essential for heat tolerance in plants. Journal of Experimental Botany 64, 1689-1701. (SCI) (IF=5.794, Ranking 11/196)
  4. Wu TM, Lin WR, Kao YT, Hsu YT, Yeh CH, Hong CY*, Kao CH (2013) Identification and characterization of a novel chloroplast/mitochondria co-localized glutathione reductase 3 involved in salt stress response in rice. Plant Molecular Biology 83, 379-390. (SCI) (IF=4.072, Ranking 19/196)
  5. Yeh CH, Kaplinsky NJ, Hu C, and Charng YY.* 2012. Some like it hot, some like it warm: Phenotyping to explore thermotolerance diversity. Plant Science 195, 10-23. (SCI) (IF=4.114, Ranking 18/196) (first author)
  6. Wang WK, Chia-Chin Liu, Chiang TY, Ming-Tse Chen, Chou CH*, and Yeh CH.* 2011. Characterization of expressed sequence tags from flower buds of alpine Lilium formosanum using a Subtractive cDNA Library. Plant Molecular Biology Reporter 29, 88-97. (SCI) (IF=2.374, Ranking 58/196) (co-corresponding author)
  7. Wang LC, Tsai MC, Chang KY, Fan YS, Yeh CH, and Wu SJ.* 2011. Involvement of the Arabidopsis HIT1/AtVPS53 tethering protein homologue in the acclimation of the plasma membrane to heat stress. Journal of Experimental Botany 62, 3609-3620. (SCI) (IF=5.794, Ranking 11/196)
  8. Guan JC, Yeh CH*, Lin YP, Ke YT, Chen MT, You JW, Liu YH, Lu CA, Wu SJ, and Lin CY.* 2010. A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses. Journal of Experimental Botany 61, 4249-4261 (SCI) (IF=5.794, Ranking 11/196) (co-first author & co-corresponding author)
  9. Huang CK, Huang LF, Huang JJ, Wu SJ, Yeh CH, and Lu CA.* 2010. A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology 51, 694-706. (SCI) (IF=4.978, Ranking 15/196)
  10. Wu SJ, Wang LC, Yeh CH, Lu CA, and Wu SJ.* 2010. Isolation and characterization of the Arabidopsis heat-intolerant 2 (hit2) mutant reveal the essential role of the nuclear export receptor EXPORTIN1A (XPO1A) in plant heat tolerance. New Phytologist 186, 833-842. (SCI) (IF=6.373, Ranking 9/196)
  11. Wang LC, *Yeh CH, Sayler RJ, Lee YY, Lu CA, and Wu SJ (2008) Arabidopsis HIT1, a putative homolog of yeast tethering protein Vps53p, is required for pollen tube elongation. Botanical bulletin of Academia Sinica 49, 25-32.
  12. *Yeh CH, Wu SJ, Chen HY, and Lin CY(2007) Physiological effects of azetidine on cellular leakage in soybean seedlings. Plant Science 171, 1124-1130.
  13. Lee CF, Pu HY, Wang LC, Sayler RJ, *Yeh CH, and Wu SJ (2006) Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant. Planta 224, 330-338.
  14. *Yeh CH, Travis D & Ho T.H.D. (2004) Expression of HVA1, a barley group III LEA protein, confers salt resistance in prokaryotic and eukaryotic cells. In preparation.
  15. Guan JC, Jinn TL, *Yeh CH, Feng SP, Chen YM, and Lin CY (2004) Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.) Plant Molecular Biology 56, 795-809.
  16. *Yeh CH, Chiu CC, Chen HI, Chen YM & Lin CY (2004) Physiological and biochemical analysis of soybean (Glycine max) small heat shock proteins induced by azetidine-2-carboxylic acid. Submitted to J of Experimental Botany
  17. *Yeh CH, Chen YM & Lin CY (2002) Functional regions of rice heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli. Plant Physiol 128, 661-668 (SCI 5.105)
  18. Chiung YM, Lin BL, *Yeh CH & Lin CY (2000) Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens. Electrophoresis 21, 297-300 (SCI 4.282).
  19. Young LS, *Yeh CH, Chen YM & Lin CY (1999) Molecular characterization of Oryza sativa 16.9 kDa heat shock protein. Biochemical J. 344, 31-38 (SCI 4.326).
  20. Lin CY, *Yeh CH, Tzeng SS, Tseng TS, Chang FC, Lee YL, Jinn TL & Chang PFL (1997) Class I low-molecualr-weight heat-shock proteins in Oryza sativa. pp41-52. In JC Su ed, Proceedings of Fifth International Symposium on Rice Molecular Biology.
  21. *Yeh CH, Chang PFL, Yeh KW, Lin WC, Chen YM & Lin CY (1997) Expression of a gene encoding a 16.9 kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance. Proc. Natl. Sci. USA 94: 10967-10972 (SCI 10.896).
  22. *Yeh CH, Yeh KW, Wu SH, Chang PFL, Chen YM & Lin CY (1995) A recombinant rice 16.9-kDa heat shock protein can provide thermotolerance in vitro. Plant Cell Physiol. 36, 1341-1348 (SCI 2.43).
  23. Yeh KW, Jinn TL, *Yeh CH, Chen YM & Lin CY (1994) Plant low-molecular-mass heat –shock proteins: their relationships to the acquisition of thermotolerance in plants. Biotechnol. Appl. Biochem. 19, 41-49 (SCI 1.408).
  24. Jinn TL, Wu SH, *Yeh CH, Hsieh MH, Yeh YC, Chen YM & Lin CY (1993) Immunological kinship of class I low-molecular-mass heat shock proteins and thermostabilization of soluble proteins in vitro among plants. Plant Cell Physiol. 34, 1055-1062 (SCI 2.43).
  25. Tseng TS, Tzeng SS, Yeh KW, *Yeh CH, Chang FC, Chen YM & Lin CY (1993) Heat shock responses in rice seedlings: Isolation and expression of cDNA encoding class I low-molecular-weight heat-shock proteins. Plant Cell Physiol. 34, 165-168 (SCI 2.43).
  26. Tseng TS, Yeh KW, *Yeh CH, Chang FC, Chen YM & Lin CY (1992) Two rice (Oryza sativa) full length cDNA clones encoding low-molecular-weight heat shock proteins. Plant Molecular Biology 18: 963-965 (SCI 3.592).

CONFERENCES

  1. Ke YT, Yeh CH. 2014. Characterization of a CONSTANS-Like gene from Phalaenopsis aphrodite subsp. formosana, PaCOL1. Annular Meeting of the American Society of Plant Physiologists.
  2. Wu CR, Liu YH, Yeh CH. 2014. Oshsp16.9A, a small heat shock protein can confer thermotolerance in rice seeds. Annular Meeting of the Botanical Society of Republic of China.
  3. Lian PJ, Wu CR, Hsu KH, Yeh CH. 2014. Analysis of the interaction between OsRZFP34, a rice RING zinc-finger protein, with its up-regulated protein. Annular Meeting of the Botanical Society of Republic of China.
  4. Wu CR, Liu YH, Yeh CH. 2013. Expression of a small heat shock protein, Oshsp16.9A, enhances thermotolerance of rice seeds. Annular Meeting of the Botanical Society of Republic of China.
  5. Gu CH, Yeh CH. 2013. Characterization of the promoter of PaCOL1, a flowering-related gene in Phalaenopsis Aphrodite. Annular Meeting of the Botanical Society of Republic of China.
  6. Ke YT, Gu CH, and Yeh CH. 2012. Functional assay of the rice group 3 late embryogenesis abundant protein genes. 10th International Congress on Plant Molecular Biology.
  7. Su SC, Wu CR, Yeh CH, and Huang LF. 2012. Manipulation of transitory starch degradation to make starch-rich rice straw for biorefinery application. 10th International Congress on Plant Molecular Biology.
  8. Yeh CH, Hsu KH, and Kuo YY. 2011. A rice RING zinc finger protein OsRZFP34 plays an essential role on mediating stomatal opening under heat stress. Annular Meeting of the American Society of Plant Physiologists.
  9. Yeh CH, Hsu KH, and Kuo YY. 2010. Physiological function assay of a heat- and abscisic acid-regulated gene, OsRZFP1. Annular Meeting of the American Society of Plant Physiologists.
  10. Hsu KH, Yeh CH, (2009) Physiological function assay of a heat- and abscisic acid-responsive rice gene, Os01g0719100. Annular Conference of Biomedical Sciences.
  11. Ke YT, Yeh CH, (2008) Characterization of rice group 3 LEA gene expression in development stages and stresses. The 6th International Symposium of Rice Functional Genomics.
  12. Chen MT, Lin, YP, Lin KF, Yeh CH, (2008) Regulation mechanism of Oshsp18.0 under treatment of L-azetidine-2-carboxylate and abiotic stresses. The 6th International Symposium of Rice Functional Genomics.
  13. Lin, YP, Chen MT, Lin KF, Yeh CH, (2008) Understanding the mechanism of HS-like response induced by denatured protein. Annular Conference of Biomedical Sciences
  14. Yeh CH, Lin, YP, Chen MT (2007) Characterization of a chemical responsive element on promoter of rice class I small heat shock protein genes. Annular Meeting of the American Society of Plant Physiologists.
  15. Lin YP, Chen MT , Yeh CH (2007) Characterization of an azatidine responsive element on promoter of rice class I small heat shock protein genes. Annular Conference of Biomedical Sciences.
  16. Yeh CH, Ting HM, and Ho TDH. Functional analysis of group 3 LEA proteins. 2006; Annular Meeting of the American Society of Plant Physiologists.
  17. Guan JC, You JW, Yeh CH,  Jinn TL, and Lin CY. A comprehensive analysis of rice small heat shock protein gene family. 2006; Annular Meeting of the American Society of Plant Physiologists.
  18. *Yeh CH, Wang , WK & (2004) Molecular evolution and expression patterns of the hemoglobin family in Arabidopsis. Annular Meeting of the American Society of Plant Physiologists.
  19. *Yeh CH, Travis D & Ho THD (2003) Expression of HVA1, a barley group III LEA protein, in Saccharomyces cerevisiae confers salt resistance. Annular Meeting of the American Society of Plant Physiologists.
  20. *Yeh CH, Chiu CC, Chen HI, Chen YM & Lin CY (2002) Physiological and Biochemical Analysis of Soybean (Glycine max) Small Heat Shock Proteins Induced by Azetidine-2-Carboxylic Acid. Annular Meeting of the American Society of Plant Physiologists.
  21. *Yeh CH, Chen YM & Lin CY (2001) Functional regions of rice (Oryza sativa) heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli. 2001 International Symposium on Plant Environment Interactions: Genes, Proteins, and Biotechnology.
  22. *Yeh CH, Chen YM & Lin CY (2001) Molecular analysis of the functional regions required for Oshsp16.9, a rice (Oryza sativa) class I low-molecular-mass heat shock protein, in relation to conferring thermotolerance. Annular Meeting of the American Society of Plant Physiologists.
  23. Young LS, *Yeh CH, Chen YM & Lin CY (1999) Molecular characterization of Oryza sativa 16.9 kDa heat shock protein. Annular Meeting of the American Society of Plant Physiologists.
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榮譽事項

  1. 1999 Dean of Science College Award, National Taiwan University

學會會員

  1. 中華植物學會
  2. 台灣中子學會
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實驗室成員

博士班學生
林冠甫 柯懿婷

碩士班學生
鄭榮儀



導生名單

年級 姓  名
大一 陳瑄敏* 呂佳蓁 洪志豪 游宜臻  
大二 林君儒 李柏寬      
大三 孔德馨 林仁豊 葉芸芷 黃昱齊  
大四 王又婷 黃畋勝 林漢莛    
延修 謝博閔        

 

 

導生照片

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