Full-time Professors

Dr. Chein-Chia Wang
王健家 博士 Position Distinguished Professor and Chair
Education Department of Biochemistry, Tulane University (LA), USA
Interest Biochemistry, Molecular Biology, Biotechnology
E-mail dukewang@cc.ncu.edu.tw
Phone 886-3-4227151 ext 65061, 65062

Personal Introduction

  1. Position: Distinguished Professor and Chair
  2. Education:
    • Tulane University (LA, USA)
      Ph. D. in Biochemistry
    • National Yang-Ming Medical College (Taipei, Taiwan)
      M.S. in Microbiology and Immunology
    • China Medical College (Taichung, Taiwan)
      B.S. in Medical Technology
  3. Professional Experience:
    • National Central University (Jung-li, Taiwan) 1/2016 ~ present
      Distinguished Professor and Chair
    • National Central University (Jung-li, Taiwan) 8/2013 ~present
      Professor and Chair
    • National Central University (Jung-li, Taiwan) 8/2009 ~present
      Professor
      Research: aaRSs and their connections to disease
    • National Central University (Jung-li, Taiwan) 8/2005 ~ 7/2009
      Associate Professor
      Research:Mechanisms of translation initiation in yeast
    • National Central University (Jung-li, Taiwan) 5/2000 ~ 7/2005
      Assistant Professor
      Research: Mechanisms of translation initiation in yeast
    • The Scripps Research Institute (CA, USA)  8/1997 ~ 4/2000
      Post-doctoral Fellow
      Research: tRNA specificity of an aaRS
    • Massachusetts Institute of Technology (MA, USA) 2/1997 ~ 7/1997
      Post-doctoral Fellow
      Research: tRNA specificity of an aaRS
  4. Current Position: Distinguished Professor and Chair
  5. Contact:886-3-422-7151 ext 65062
  6. FAX:(03) 422-8482
  7. E-mail:dukewang@cc.ncu.edu.tw

Teaching

  1. Biochemistry
  2. Biochemistry Laboratory
  3. Gene Regulation
  4. Gene Cloning

Research project

Aminoacyl-tRNA synthetases and their connections to disease

Faithful decoding of mRNA depends on accurate aminoacylation of tRNA (aa + tRNA → aa-tRNA) by aminoacyl-tRNA synthetases (aaRSs) and a specific readout of the codons by tRNAs. AaRSs are ubiquitously expressed, essential enzymes, each of which catalyzes the ligation of a specific amino acid to its cognate tRNA. The resultant aminoacyl-tRNA is then delivered by EF-Tu (or eEF1 in eukaryotes) to ribosomes for protein translation.

Lab members

Post-doc                 Teresa Chang
Research assistant  A-Mei Lo
PhD student
Yi-Hsueh Li
Cynthia Chien
Adam Tseng
Master student      
Vivian Chen (Taiwan)
Indra Lasmana Tarigan (Indonesia) (indratarigan92@gmail.com)
Duong Ngoc Kieu Thi (Vietnam) (duongngockieuthi@gmail.com)
Restu Nugraha (Indonesia) (Restunugraha10@gmail.com)
Nguyen Viet Khang (Vietnam) (vietkhangnguyen92@gmail.com)
Tran Hanh Phuoc (Vietnam) (ptrnhanh@gmail.com)
Fanny Fan (Taiwan)
Undergraduate   6~8 special topic undergraduates

Publications (Referred papers)

  1. Lee, Y. H., Chang, C. P., Cheng, Y. J., Kuo, Y. Y., Lin, Y. S., and Wang, C. C.* (2017) Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase. Cell. Mol. Life Sci. (Epub ahead of print) (SCI) (MOST 103-2311-B-008-003-MY3, MOST 103-2923-B-008-001-MY3, and NSC 102-2311-B-008-004-MY3)
  2. Chang, C. Y., Chang, C. P., Chakraborty, S., Wang, S. W., Tseng, Y. K., Wang, C. C.* (2016) Modulating the structure and function of an aminoacyl-tRNA synthetase cofactor by biotinylation. J. Biol. Chem. 291: 17102-17111 (SCI) (MOST 103-2311-B-008-003-MY3, MOST 103-2923-B-008-001-MY3, and NSC 102-2311-B-008-004-MY3)
  3. Chang, C. Y., Chien, C. I., Chang, C. P., Lin, B. C., and Wang, C. C.* (2016) A WHEP domain regulates the dynamic structure and activity of Caenorhabditis elegans glycyl-tRNA synthetase. J. Biol. Chem. 291: 16567-16575 (SCI) (MOST 103-2311-B-008-003-MY3, MOST 103-2923-B-008-001-MY3, NSC 102-2311-B-008-004-MY3, and NCU-LSH-103-A-003)
  4. Chang, C. P., Chang, C. Y., Li, Y. X., Lin, Y. S., and Wang, C. C.* (2015)Divergent alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora descended from a common ancestor through whole-genome duplication followed by asymmetric evolution. Mol. Cell. Biol. 35: 2242-2253 (SCI) (MOST 103-2311-B-008-003-MY3, MOST 103-2923-B-008-001-MY3, NSC102-2311-B-008-004-MY3, and NCU-LSH-103-A-003)
  5. Chien, C. I., Chen, Y. L., Chen, S. J., Chou, C. M., Chen, C. Y., and Wang, C. C.* (2015) Vanderwaltozyma polyspora possesses two glycyl-tRNA synthetase genes: one constitutive and one inducible. Fungal Genet. Biol. (accepted) (SCI) (NSC102-2311-B-008-004-MY3) (IF=3.3; R/C=4/23, MYCOLOGY)
  6. Chien, C. I., Chen, Y. W., Wu, Y. H., Chang, C. Y., Wang, T. L., and Wang, C. C.* (2014) Functional substitution of a eukaryotic glycyl-tRNA synthetase with an evolutionarily unrelated bacterial cognate enzyme. PLoS ONE 9: e94659. (IF=3.7; R/C=7/56, MULTIDISCIPLINARY SCIENCES)
  7. Wu, Y. H., Chang, C. P., Chien, C. I., Tseng, Y. K., and Wang, C. C.* (2013) An insertion peptide in yeast glycyl-tRNA synthetase facilitates both productive docking and catalysis of cognate tRNAs. Mol. Cell. Biol. 33: 3515-3523 (SCI) (NSC101-3113-B-008-001) (IF=5.4; R/C=50/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  8. Liao, C. C., Lin, C. H., Chen, S. J., and Wang, C. C.* (2012) Trans-kingdom rescue of Gln-tRNAGln synthesis in yeast cytoplasm and mitochondria. Nucleic Acids Res. 40: 9171-9181 (SCI) (NSC101-3113-B-008-001) (IF=8.3; R/C=27/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  9. Chen, S. J., Wu, Y. H., Huang, H. Y., and Wang, C. C.* (2012) Saccharomyces cerevisiae possesses a stress-inducible glycyl-tRNA synthetase gene. PLoS ONE 7: e33363. (SCI) (NSC97-2311-B-008-002-MY3, NSC97-2311-B-008-003-MY3, and NSC100-3114-B-008-001-MY3) (IF=3.7; R/C=7/56, MULTIDISCIPLINARY SCIENCES)
  10. Chang, C. P., Tseng, Y. K., Ko, C. Y., and Wang, C. C.* (2012) Alanyl-tRNA synthetase genes of Vanderwaltozyma polyspora arose from duplication of a dual-functional predecessor of mitochondrial origin. Nucleic Acids Res. 40: 314-322. (SCI) (NSC97-2311-B-008-002-MY3 and NSC97-2311-B-008-003-MY3) (IF=8.3; R/C=27/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  11. Chen, S. J., Lee, C. Y., Lin, S. T., and Wang, C. C.* (2011) Rescuing a dysfunctional homologue of a yeast glycyl-tRNA synthetase gene. ACS Chem. Biol. 6: 1182-1187. (SCI) (NSC97-2311-B-008-003-MY3 and NSC98-3114-B-008-002) (IF=5.4; R/C=48/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  12. Lin, C. H., Lin, G., Chang, C. P., and Wang, C. C.* (2010) A tryptophan-rich peptide acts as a transcription activation domain. BMC Mol. Biol. 11: 85. (SCI) (NSC97-2311-B-008-003-MY3) (IF=2.8; R/C=147/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  13. Chang, C. P., Chen, S. J., Lin, C. H., Wang, T. L., and Wang, C. C.* (2010) A single sequence context cannot satisfy all non-AUG initiator codons in yeast. BMC Microbiol. 10: 188. (SCI) (NSC97-2311-B-008-003-MY3) (The first two authors contributed equally to this work) (IF=3.1; R/C=41/116, MICROBIOLOGY)
  14. Chiu, W. C., Chang, C. P., Wen, W. L., Wang, S. W., and Wang, C. C.* (2010) Schizosaccharomyces pombe possesses two paralogous valyl-tRNA synthetase genes of mitochondrial origin. Mol. Biol. Evol. 27: 1415-1424. (SCI) (NSC96-2311-B-008-002) (IF=10.4; R/C=14/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  15. Chiu, W. C., Chang, C. P., and Wang, C. C.* (2009) Evolutionary basis of converting a bacterial tRNA synthetase into a yeast cytoplasmic or mitochondrial enzyme. J. Biol. Chem. 284: 23954-23960 (SCI) (NSC97-2311-B-008-002-MY3) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  16. Chen, S. J., Ko, C. Y., Yen, C. W., and Wang, C. C.* (2009) Translational efficiency of redundant ACG initiator codons is enhanced by a favorable sequence context and remedial initiation. J. Biol. Chem. 284: 818-827 (SCI) (NSC96-2311-B-008-002, NSC96-2311-B-008-003, and CNJRF-96CGH-NCU-B4) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  17. Chang, C. P., Lin, G., Chen, S. J., Chiu, W. C., Chen, W. H., and Wang, C. C.* (2008) Promoting the formation of an active synthetase/tRNA complex by a non-specific tRNA-binding domain. J. Biol. Chem. 283: 30699-30706 (SCI) (NSC 96-2311-B-008-002 and 96-2001-INER-0034) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  18. Chen, S. J., Lin, G., Chang, K. J., Yeh, L. S., and Wang, C. C.* (2008) Translational efficiency of a non-AUG initiation codon is significantly affected by its sequence context in yeast. J. Biol. Chem. 283: 3173-3180 (SCI) (NSC 95-2311-B-008-005 and NSC 95-2311-B-008-006) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  19. Huang, H. Y., Kuei, Y., Chao, H. Y., Chen, S. J., Yeh, L. S., and Wang, C. C.* (2006) Cross-species and cross-compartmental aminoacylation of isoaccepting tRNAs by a class II tRNA synthetase. J. Biol. Chem. 281: 31430-31439 (SCI) (NSC 94-2311-B-008-009 and INER 94-2001-INER-EE-009) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  20. Huang, H. Y., Tang, H. L., Chao, H. Y., Yeh, L. S., and Wang, C. C.* (2006) An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms. Mol. Microbiol. 60(1): 189-198 (SCI) (NSC 93-2311-B-008-001) (IF=5.0; R/C=56/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  21. Chang, K. J., Grace, L., Men, L. C., and Wang, C. C.* (2006) Redundancy of non-AUG initiators: a clever mechanism to enhance the efficiency of translation in yeast. J. Biol. Chem. 281: 7775-7783 (SCI) (NSC 94-2311-B-008-009 and INER 94-2001-INER-EE-009) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  22. Tang, H. L., Yeh, L. S., Chen, N. K., Ripmaster, T., Schimmel, P., and Wang, C. C.* (2004) Translation of a yeast mitochondrial tRNA synthetase initiated at redundant non-AUG codons. J. Biol. Chem. 279: 49656-49663 (SCI) (NSC 92-2311-B-008-008) (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  23. Chang, K. J. and Wang, C. C.* (2004) Translation initiation from a naturally occurring non-AUG codon in Saccharomyces cerevisiae. J. Biol. Chem. 279: 13778-13785  (SCI) (NSC 91-2311-B-008-008)(IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  24. Wang, C. C.*, Chang, K. J., Tang, H. L., Hsieh, C. J., and Schimmel, P. 2003 Mitochondrial form of a tRNA synthetase can be made bifunctional by manipulating its leader peptide. Biochemistry 42: 1646-1651 (SCI) (NSC 90-2311-B-008-004) (IF=3.4; R/C=102/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  25. Wang, C. C., Morales, A., and Schimmel, P. 2000 Functional redundancy in the nonspecific RNA binding domain of a class I tRNA synthetase. J. Biol. Chem. 275: 17180-17186 (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  26. Houman, F., Rho, S. B., Zhang, J., Shen, X., Wang, C. C., Schimmel, P., and Martinis, S. A. 2000 A prokaryote and human tRNA synthetases provide an essential RNA splicing function in yeast mitochondria. Proc. Natl. Acad. Sci.  97: 13743-13748 (IF=9.7; R/C=4/56, MULTIDISCIPLINARY SCIENCES)
  27. Swairjo, M., Morales, A., Wang, C. C., Ortiz, A. R., and Schimmel, P. 2000 Crystal structure of Trbp111: a structure-specific tRNA binding protein. EMBO J.19: 6287-6298 (IF=9.8; R/C=17/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  28. Schimmel, P. and Wang, C. C. 1999 Getting tRNA synthetases into the nucleus. Trends Biochem. Sci. 24: 127-128 (IF=13.1; R/C=8/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  29. Wang, C. C. and Schimmel, P. 1999 Species barrier to RNA recognition overcome with nonspecific RNA binding domains. J. Biol. Chem.274: 16508-16512 (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  30. Dressman, H. K., Wang, C. C., Karam, J. D., and Drake, J. W. 1997 Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment. Proc. Natl. Acad. Sci. 94: 8042-8046 (IF=9.7; R/C=4/56, MULTIDISCIPLINARY SCIENCES)
  31. Wang, C. C., Pavlov, A., and Karam, J. D. 1997 Evolution of RNA-binding specificity in T4 DNA polymerase. J. Biol. Chem.272: 17703-17710 (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  32. Wang, J., Sattar, A. K., Wang, C. C., Karam, J. D., Konigsberg, W. H., and Steitz, T. A. 1997 Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69. Cell 89: 1087-1099 (IF=32.0; R/C=1/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)
  33. Wang, C. C., Yeh, L. S., and Karam, J. D. 1995 Modular organization of T4 DNA polymerase. Evidence from phylogenetics. J. Biol. Chem. 270: 26558-26564 (IF=4.7; R/C=62/290, BIOCHEMISTRY & MOLECULAR BIOLOGY)

Honors and Awards obtained by the lab members (2005 ~ present)

  1. Master Thesis Award (to Kuang-Jung Chang, graduate student) from the National Science Council (2003)
  2. Excellent Thesis Award (to Kuang-Jung Chang, graduate student) from the Academic Foundation of Professor Jong-Iou Lin (2004)
  3. Taiwan Merit Scholarships (to Kuang-Jung Chang, graduate student) from NSC, ME, and CEPD (2005)
  4. Excellent Poster Award (to Hsiao-Yun Huang, graduate student) from the 21st Joint Biomedical Sciences Conference (2006)
  5. Research Outstanding Award for Graduate Students (to Shun-Jia Chen, graduate student) from National Central University (2006)
  6. Excellent Thesis Award (to Hsiao-Yun Huang, graduate student) from the Academic Foundation of Professor Jong-Iou Lin (2006)
  7. Taiwan Merit Scholarships (to Hsiao-Yun Huang, graduate student) from NSC, ME, and CEPD (2006)
  8. Excellent Poster Award (to Chia-Pei Chang, graduate student) from the 22nd Joint Biomedical Sciences Conference (2007)
  9. Excellent Poster Award (to Shun-Chia Chen, graduate student) from the Sixteenth Symposium on Recent Advances in Cellular and Molecular Biology (2008)
  10. Excellent Poster Award (to Shun-Jia Chen, Ph.D. student) from the Joint Conference of National Central University and Chung Yuan Christian University on Biological Sciences (2008)
  11. Research Outstanding Award for Graduate Students (to Shun-Jia Chen, Ph.D. student) from National Central University (2008)
  12. Presidential Scholarship Award (to Shun-Jia Chen, Ph.D. student) from National Central University (2009)
  13. Research Outstanding Award for Graduate Students (to Chia-Pei Chang, Ph.D. student) from National Central University (2009)
  14. Presidential Scholarship Award (to Wen-Chi Chiu, Ph.D. student) from National Central University (2010)

Awards and Activities

  1. Outstanding Teacher Award, College of Science, NCU (2001)
  2. Junior Research Investigator Award from Academia Sinica (2005)
  3. Outstanding Research Award, National Central University (2005)
  4. Outstanding Research Award, National Central University (2006)
  5. Top research article award, College of Science, National Central University (2006)
  6. Outstanding Research Award, National Central University (2007)
  7. Outstanding Research Award, National Central University (2008)
  8. Top research article award, College of Science, National Central University (2009)
  9. Outstanding Teacher Award, College of Science, NCU (2009)
  10. Outstanding Research Award, National Central University (2010)
  11. Outstanding Research Award, National Central University (2011)
  12. Top research article award, College of Science, National Central University (2012)
  13. Outstanding Research Award, National Central University (2012)
  14. Outstanding Research Award, National Central University (2014)
  15. Distinguished Professor Award, National Central University (2016)

Affiliations

  1. American Society for Biochemistry and Molecular Biology, USA (2005 ~ present)
  2. American Society for Microbiology (2014~ present)
  3. The Chinese Society of Cell and Molecular Biology (2001 ~ present)
  4. Taiwanese Society for Genomics and Genetics (2006 ~ present)
  5. The Society for Experimental Biology, UK (2009)