專任師資

吳少傑 博士
吳少傑 博士 職稱: 教授
學歷: 美國奧本大學
專長: 植物生理學、DNA運算法、植物逆境、生物科技
信箱: jyewu@cc.ncu.edu.tw
分機: 65052, 65072

個人介紹

  1. 職 稱:教授
  2. 學 歷:
    1. Ph. D. Department of Botany and Microbiology Auburn University, AL 36849 Dissertation : Molecular genetic approach to the study of stress reponases in Arabidopsis. Dec. 1997
    2. M.S. Department of Botany and Microbiology Auburn University, AL 36849 Thesis title : Search for sigma factor 54 in Frankia .Aug. 1994
    3. B.A. Department of Botany National Taiwan University Taipei, Taiwan Jul. 1989
  3. 經 歷:
    1. 2002-2011/7 國立中央大學 生命科學研究所 助理教授
    2. Postdoctoral Research Associate (2000 - to 2002) Plant Pathology Department, University of California at Davis Project: "Application of DNA macro array in the detection and identification of pathogens"
    3. Postdoctoral Research Associate (1998 - 2000) Department of Biological Sciences, Auburn University Project: "Regulation of Arabidopsis Glutamate Decarboxylase (GAD) in Response to Heat Stress."
  4. 現 職: 2015/8-present 國立中央大學 生命科學研究所 教授
  5. 個人網頁: http://www.ncu.edu.tw/~jyewu/
  6. 聯絡方式:(03)422-7151 (分機 65052)
  7. FAX:(03) 422-8482
  8. E-mail:jyewu@cc.ncu.edu.tw

授課

  1. 質體學
  2. 植物生理學
  3. 生物科技實例研討
  4. 計算機概論
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研究領域

對抗因全球暖化所造成糧食減產的威脅 --
運用前向式遺傳學之策略研究植物耐熱機制

 

植物因為無法自由行動,故其生長會時刻受到環境的影響。而由於地球溫室效應的擴大,加之世界人口的增家,可耕地面積的減少,了解植物對抗高溫逆境的機制,進而找出提高農作物耐熱能力的途逕, 已成為世界各國必須且迫切需要面對的課題。而在諸多研究策略之中,又以找出植物負責抵抗高溫逆境的基因,以利遺傳工成之便,最受重視。

<<過去十年地表均溫變化的情形。紅色越深的區域,暖化現像越嚴重。>>

要找出植物抵抗高溫逆境的遺傳因子,最通用的方法是經由基因體(genomics)或蛋白質體(proteomics)學,找出受高溫誘導表達的基因。利用這種方法,植物學家們已經獲得不少寶貴的資料,例如熱休克蛋白的基因表現及其在細胞分子生物學上的角色。然而,單是利用 基因體學及蛋白質體學的方式,並無法找出所有抵抗高溫逆境的基因。因為有許多基因的表現,雖不受高溫所誘導或抑制,但它們的產物卻是植物抵抗高溫逆境所不可或缺的。例如負責訊號感應,和訊號傳遞的基因。

很顯然的,必須有其它的研究策略,才能涵蓋現有的不足。因此,我們採用前向式遺傳學的方法 (forward genetics approach),來突破這個困境。當植物內部負責抵禦高溫逆境的基因,因突變而喪失功能,帶有此突變基因的植物,就會對高溫逆境失去耐受性(敏感型)。藉由對高溫逆境敏感型突變植 株的篩選,我們不但可以找出植物抵抗高溫逆境的遺傳因子,該遺傳因子的功能與角色,也可從對該突變植物的生理研究得到解答。

應用上述原理及方法,本實驗室已成功的從模式植物阿拉伯芥(Arabidopsis thaliana),篩選出若干對高溫逆境過度敏感的突變種植物。經由基因定位, 及後續的生理、細胞、遺傳、乃至於分子生物的實驗,至今我們已發現三個前所未知的植物耐熱基因(我們將它們命名為HIT1、HIT2及HIT4) ,並解構出它們保護植物免於高溫傷害的機制 (詳細內容,請到http://www.ncu.edu.tw/~jyewu/RI/index.htm 參閱)。

<<W, 野生型;h, hit1 突變型; T, 將野生型HIT1 基因殖入hit1 突變型之轉殖株。幼苗在23°C萌發10天後移至37oC培育4天,照片攝於37oC培育前與培育後。>>

除了hit1 hit2 hit4 之外,本實驗室 還有許多熱敏感突變株未被研究。我們相信,每個突變株背後都有一個令人驚奇讚歎的道理。這部份正等待著有興趣及企圖心的新血,加入我們的團對,開創新局面。

 

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論文發表

期刊

  1. Wu, J.-R., Wang, L.-C., Lin, Y.-R, Weng, C.-P., Yeh, C.-H., Wu, S.-J.* (2016) The Arabidopsis heat-intolerant 5 (hit5)/enhanced response to aba 1 (era1) mutant reveals the crucial role of protein farnesylation in plant responses to heat stress. New Phytologist (SCI) in press.
  2. Ke, Y.-T., Lu, C.-A., Wu, S.-J., Yeh, C.-H. (2016) Characterization of rice group 3 LEA genes in developmental stages and under abiotic stress. Plant Molecular Biology Reporter .34:1003-1015. (SCI)
  3. Huang, C.-K., Shen, Y.-L., Huang, L.-F., Wu, S.-J., Yeh, C.-H., Lu, C.-A. (2016) A single-repeat MYB transcription represor, The DEAD-box RNA helicase AtRH7/PRH75 participates in pre-rRNA processing, plant development and cold tolerance in Arabidopsis. Plant and Cell Physiology 57:174-191. (SCI)
  4. Wang, L.-C., Wu, J.-R., Hsu, Y.-J, Wu, S.-J.* (2015) Arabidopsis HIT4, a regulator involved in heat-triggered reorganization of chromatin and release of transcriptional gene silencing, relocates from chromocenters to the nucleoculs in response to heat stress. New Phytologist 205:544-554 (SCI)
  5. Huang, C.-K., Lo, P-C., Huang, L.-F., Wu, S.-J., Yeh, C.-H., Lu, C.-A. (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Molecular Biology 88:269-286 (SCI)
  6. Hsu K.-H., Liu C.-C., Wu, S.-J., Kuo, Y.-Y., Lu, C.-A., Wu, C.-R., Lian, P.-J. Hong, C.-Y., Ke, Y.-T., Huang, J.-H., Yeh, C.-H.* (2014) Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening. Plant Molecular Biology 86:125-137.(SCI)
  7. Chou, W.-L., Huang, L.-F., Fang, J.-C., Yeh, C.-H., Hong, C.-Y., Wu, S.-J., Lu, C.-A. (2014). Divergence of the expression and subcellular localization of CCR4-associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Molecular Biology 85:443-458 (SCI)
  8. Wang, L.-C., Wu, J.-R., Chang, W.-L., Yeh, C.-H., Ke, Y.-T., Lu, C.-A., Wu, S.-J.* (2013) Arabidopsis HIT4 encodes a novel chromocentre-localized protein involved in the heat reactivation of transcriptionally silent loci and is essential for heat tolerance in plants. Journal of Experimental Botany 64:1689-1701 (SCI)
  9. Chen, I.-J., Lo, W.-S., Chaung, J.-Y., Cheuh, C.-M., Fan, Y.-S., Lin, L.-C., Wu, S.-J., Wang, L.-C.* (2013) A chemical genetic approach reveals a role of brassinolide and cellulose synthase in hypocotyl elongation of etiolated Arabidopsis seedlings. Plant Science 209:46-57 (SCI)
  10. Wang, L.-C., Tsai M.-C., Chang K.-Y., Fan, Y.S., Yeh, C.-H., Wu, S.-J*. (2011) Involvement of the Arabidopsis HIT1/AtVPS53 tethering protein homologue in the acclimation of the plasma membrane to heat stress. Journal of Experimental Botany 62:3209-3612 (SCI)
  11. Guan J-C, Yeh C-H, Lin Y-P, Ke Y-T, Chen M-T, You J-W, Liu Y-H, Lu C-A, Wu S-J, Lin CY*. (2010) A 9bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses. Journal of Experimental Botany 61:4249-4261 (SCI)
  12. Huang, C.-K., Huang, L.-F., Huang, J.-J., Wu, S.-J., Yeh, C.-H., Lu, C.-A. (2010) A DEAD-box protein, AtRH36, Is Essential for Female Gametophyte Development and Is Involved in rRNA Biogenesis in Arabidopsis. Plant Cell and Physiology 51:694-706 (SCI)
  13. Wu, S.-J., Wang, L.-C, Yeh, C.-H., Lu, C.-A., Wu, S.-J.* (2010) Isolation and characterization of the Arabidopsis hit-intolerant 2 (hit2)mutant reveal the essential role of the nuclear export receptor EXPORTIN1A (XOP1A) in plant heat tolerance. New Phytologist 186: 833-842 (SCI)
  14. Wang, L.-C, Yeh, C.-H., Sayler, R.J., Lee, Y.-Y, Lu C.-A., Wu, S.-J.* (2008) Arabidopsis HIT1, a putative homolog of yeast tethering protein Vps53p, is required for pollen tube elongation. Botanical Studies 49:25~32 (SCI)
  15. Yeh, C.-H., Wu, S.-J., Tsai, Y.-F., Chen, H.-Y., Lin, C.-Y.* (2007) Physiological effects of azetidine on cellular leakage in soybean seedlings. Plant Science 172: 1124-1130 (SCI)
  16. Lee, C-F., Pu, H.-Y., Wang, L.-C, Sayler, R. J., Yeh, C.-H, Wu, S.-J.* (2006) Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant. Planta 224:330-338.
  17. Pu, H.-Y., Wu, S.-J.* (2005) A microtray-based aggregation assay for the rapid detection of PCR amplicons produced from bacterial pathogens. Journal of Rapid Methods and Automation in Microbiology 13: 257-268.
  18. Wu, S.-J. and Kado, C.I. (2004) Preparation of milk samples for PCR analysis using a rapid filtration technique. Journal of Applied Microbiology, 96:1342-1346.
  19. Wu, S.-J., Chan, A. and Kado, C.I. (2004) Detection of PCR amplicons from bacterial pathogens using microsphere agglutination. Journal of Microbiological Methods, 56:395-400.
  20. Shi, H., Lee, B. Wu, S.-J. and Zhu, J.-K. (2003) Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana. Nature Biotechnology, 21: 81-85.
  21. Wang, S.-Y., Wu, S.-J., Thottappilly, G., Locy, R. D., and Singh, N. K. (2001) Molecular cloning and structural analysis of the gene encoding Bacillus cereus exochitinase Chi36. J Bioscience and Bioengineering, 92 (1) 59-66.
  22. Wang, S.-Y., Moyne, A.-L., Thottappilly, G., Wu, S.-J., Locy R. D., and Singh, N. K. (2001) Purification and Characterization of a Bacillus cereus exochitinase. Enzyme and Microbial Technology, 28 (6) 492-498.
  23. Wu, S.-J., Locy, R. D., Shaw, J. J., Cherry, J. H. Singh, N. K. (2000) Mutation in Arabidopsis HIT1 locus causing heat and osmotic hypersensitivity. J Plant Physiol, 157 (5) 543-547.
  24. Locy, R.D., Wu, S.-J., Bisnette, J., Barger, W., McNabb, D., Zik, M., Fromm, H., Singh, N.K., and Cherry, J.H. (2000) The regulation of GABA accumualtion by heat stress in Arabidopsis. In Plant Tolerance to Abiotic Stresses in Agriculture: Role of Genetic Engineering. J.H. Cherry, R.D. Locy and A. Rychter, eds. Kluwer Academic Publishers, Dordrecht, pp39-53.
  25. Shaw, J. J., Ramaika, C. A., Hawkins, L. K., Wu, S.-J., Beninga, K., McCleskey, F., DelVecchio, V. (1997) Detection of PCR amplicons diagnostic of E. coli O157:H7 with the Universal GeneComb? kit. Bio-Red US Bulletin 2161.
  26. Wu, S.-J., Wu, Y.-J. J., and Shaw, J. J. (1997). DNA solutions of weighted cycle searching problems. J. Ala. Acad. Sci. 68 (3) 259-271.
  27. Wu, S.-J., Wu, Y.-J. J., Shaw, J. J. (1996) A comprehensive DNA arithmetic calculator. Mathematics and Computer Science Division Preprint ANL/MCS-p630-1296, Argonne National Laboratory, IL.
  28. Wu, S.-J., Ding, L., Zhu, J.-K. (1996) SOS1, a genetic locus essential for salt tolerance and potassium acquisition. Plant Cell. 8(4) 617-627.
  29. Shaw, J. J., Wu, S.-J., Singh, N. P., Mahaffee, W., Dane, F., Brown, A. E. (1996) Antibiotic, Chromogenic, and luminescent markers for bacteria. In C. J. Hurst, G. R. Knudsen, M. J. McInerney, L. D. Stetzenbach and M. V. Walter, eds, Manual of Environmental Microbiology, ASM Press, Washington D.C., pp. 466-472.
  30. Wu, S.-J., Shaw, J. J., Cherry, J. H., Zhu, J.-K. (1995) Isolation and characterization of salt hypersensitive mutants of Arabidopsis thaliana." Plant Physiol. 108(2), p.59.

專利

  1. 美國發明專利
    Patent No.: US 8,962,919 B2
    Method for enhancing thermotolerance of plant relating to
    EXPORTIN1A and genetic engineering application thereof
    核准日期:2015-02-24

  2. A method for enhancing heat tolerance in plants
    Taiwan Patent (Invention) No. I441591
    From 2014/06/21 ~ 2030/09/06
    使植物表現後天性高溫逆境耐受性狀的方法及其應用。
    中華民國新發明專利 I441591

  3. 專利名稱:基因的檢測方法
    發明人:吳少傑
    專利權人:中央大學
    國別:中華民國
    專利核准日期:100.2.12

書籍

  1. Lian-Chin Wang, and Shaw-Jye Wu. The EXPORTIN1 gene (XPO1A and XPO1B) in Arabidopsis- Are they functionally redundant? In Nuclear Receptors (Margaret K. Bates et al, ed.), Nova Science Publishers Chepter7.

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榮譽事項

  1. 2011年中央大學研究傑出獎
  2. 2013年中央大學研究傑出獎
  3. 2006年發表於 Planta 期刊之論文,獲國際生物學論文評選組織 Faculty of 1000 Biology 推薦為 must read article
  4. 94學年 理學院優良教師
  5. 94學年 全校優良導師
  6. 93學年 全校優良導師
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實驗室成員

助理研究學者
研究助理
博士班學生
鄭凱恩 唐蒂琪

碩士班學生
羅予苡 王孜勻
王妍菱  

 

導生照片

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